Associate Professor Tzu-Pin Wang


王子斌

Tzu-Pin Wang, Ph.D.

Office: N818 (behind Rm N845)                                                           

Laboratory: N845, Nucleic Acid Chemistry Laboratory

Phone: 07-3121101 ext.2756

E-mail: tzupinw@kmu.edu.tw

Education

  1. National Taiwan University, B.S. in Forestry(1989)。
  2. California State University, Fullerton; CSUF; M.S. in Biochemistry(1994)。
  3. State University of New York, College of Environmental Science and Forestry; SUNY-ESF; Ph.D. in Chemistry (2000.12)

Research Highlights

  1. Chemistry of ribozymes and aptamers: in vitro evolution and selection of novel ribozymes and aptamers; structure determination of ribozymes and aptamers; determination of catalytical mechanisms of ribozymes.
  2. Nucleic acid chemistry and applications: phosphoramidation chemistry for post-synthetic modifications and conjugations of DNA/RNA; novel and effective bioorthogonal chemical approaches for nucleic acid modifications and labeling; novel approaches for synthesis of peptide-oligonucleotide conjugates (POCs); synthesis of POCs for achieving gene silencing and RNA interference ; novel methods for universal labeling and modifications DNA/RNA; phosphoramidation reactions for DNA microarray fabrication and detection; novel ribozymes and aptamers for developments of nucleic acid-based biosensors.
  3. Agarose Chemistry: purification of biotechnological grade agarose from indigenous Rhodophyta in Taiwan; synthesis of agarose derivatives useful in biotechnology and bioengineering.

Experience

  1. Kaohsiung Medical University, associate professor (2013.02-now).
  2. Kaohsiung Medical University, assistant professor (2005~2013)
  3. SUNY, University at Buffalo, Department of Chemistry, postdoctoral research associate(2000~2002.
  4. SUNY, University at Buffalo, Department of Chemistry, postdoctoral research associate(2003~2005.
  5. SUNY-ESF, Department of Chemistry, teaching and research assistants.
  6. CSUF, Department of Chemistry and Biochemistry, teaching and research assistants.

Patents:

1.       Wang, T.-P. (王子斌) and Y.-J. Chiou (邱奕璋) (2014) Methods for conjugating nucleic acids with small molecules, U.S. Patent No.US 8,765,934 B2, Date of Patent: Jul. 1, 2014.

2.      Wang, T.-P. (王子斌) and Y.-J. Chiou (邱奕璋) (2014)將核酸街上小分子的方法(Methods for conjugating nucleic acids with small molecules), Taiwan Patent No.I464402, Date of Patent: Dec. 11, 2014.

3.      Wang, T.-P. (王子斌), N. C. Ko (柯霓蒨), and Y.-C. Su (蘇郁智) (2015)核酸共軛物的液相合成法(Liquid phase synthesis of a nucleic acid conjugate), Taiwan Patent No.I471331, Date of Patent: Feb. 1, 2015.

4.      Wang, T.-P. (王子斌)and Y.-C. Su (蘇郁智) (2018) Reagents for universal site-specific labeling and modifications of nucleic acids, U.S. Patent No.: US 9,856,285 B2, Date of Patent: Jan. 02, 2018.

 

 

Journal Publications in the Past Ten Years:

1. Wang, T.-P. *, Y.-C. Su, Y. Chen, S. Severance, C.-C. Hwang, Y.-M. Liou, C.-H. Lu, K.-L. Lin, R. J. Zhu, E.-C. Wang. (2018) Corroboration of Zn(II)-Mg(II)-tertiary structure interplays essential to optimal catalysis of a phosphorothiolate thiolesterase ribozyme. RSC Adv.8, 32775-32793.

2. Hwang, C.-C.*, P.-R. Chang, T.-P. Wang. (2017) Contribution of remote substrate binding energy to the enzymatic rate acceleration for 3α-hydroxysteroid

3. Su, Y.-C., Y.-L. Lo, C.-C. Hwang, L.-F. Wang, M. H. Wu, E.-C. Wang, Y.-M. Wang, T.-P. Wang*. (2014) Azide–alkyne cycloaddition for universal post-synthetic modifications of nucleic acids and effective synthesis of bioactive nucleic acid conjugates. Org. Biomol. Chem., 12, 6624–6633.

4. Su, Y.-C., H.-Y. Chen, N. C. Ko, C.-C. Hwang, M. H. Wu, L.-F. Wang, Y.-M. Wang, S.-N. Chang, E.-C. Wang*, T.-P. Wang*. (2014) Effective and site-specific phosphoramidation reaction for universally labeling nucleic acids. Anal. Biochem.449, 118-128.

5. Chang, Y.-T., C.-Y. Lin, P.-L. Lu, C.-C. Lai, T.-C. Chen, C.-Y. Chen, D.-C. W, T.-P. Wang, C.-M. Lin, W.-R. Lin*, Y.-H. Chen. (2014) Stenotrophomonas maltophilia bloodstream infection: Comparison between community-onset and hospital-acquired infections. J. Microbiol. Immunol. Infect.47, 28-35.

6. Huang, S.-J., T.-P. Wang, S.-I. Lue, L.-F. Wang*. (2013) Pentablock copolymers of pluronic F127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies. Int. J. Nanomedicine8, 2011–2027.

7. Hwang, C.-C.*, Y.-H. Chang, H.-J. Lee, T.-P. Wang, Y.-M. Su, H.-W. Chen, P.-
H. Liang. (2013) The Catalytic Roles of P185 and T188 and Substrate-Binding Loop Flexibility in 3a-Hydroxysteroid Dehydrogenase/Carbonyl Reductase from Comamonas testosterone. PLOS ONE8, e63594.

8. Wu, S.-C., K.-L. Lin, T.-P. Wang, S.-C. Tzou, G. Singh, M.-H. Chen, T.-L. Cheng, C.-Y. Chen, G.-C. Liu, T.-W. Lee, S.-H. Hu, and Y.-M. Wang*. (2013) Imaging specificity of MR-optical imaging agents following the masking of surface charge by poly(ethylene glycol). Biomaterials34, 4118-4127.

9. Hsu, S. C. N., T.-P. Wang, C.-L. Kao, H.-F. Chen, P.-Y. Yang, and H.-Y. Chen*. (2013) Theoretical study on the protonation of the one-electron reduced guanine-cytosine base pair by water. J. Phys. Chem. B117, 2096−2105.

10. Chen, P.-Y., C.-Y. Zhou, H.-M. Chen, C.-H. Yang, T.-P. Wang*, and E.-C. Wang*. (2013) Synthesis of novel four-fused-ring chromeno-benzoxepinones from salicylaldehydes and 1-benzoxepin-5-ones via the oxa-Michael reaction/aldol condensation. ARKIVOC, (iii) 24-35.

11. Chen, P.-Y., Y.-H. Wu, M.-H. Hsu, T.-P. Wang, E.-C. Wang*. (2013) Cerium ammonium nitrate-mediated the oxidative dimerization of p-alkenylphenols: a new synthesis of substituted (±)-trans-dihydrobenzofurans. Tetrahedron69. 653-657

12. Wang, T.-P.*, N. C. Ko, Y.-C. Su, E.-C. Wang, S. Severance, C.-C. Hwang, Y. T. Shih, M. H. Wu and Y.-H. Chen*. (2012) Advanced aqueous-phase phosphoramidation reactions for effectively synthesizing peptide-oligonucleotide conjugates trafficked into a human cell line. Bioconjugate Chem.23, 2417-2433.

13. Chen,S.-H., Y.-T. Kuo, G. Singh, T.-L. Cheng, Y.-Z. Su, T.-P. Wang,
Y.-Y. Chiu, J.-J. Lai, C.-C. Chang, T.-S. Jaw, S.-C. Tzou, G.-C. Liu*, and Y.-M. Wang*. (2012) Development of a Gd(III) Based Receptor-Induced Magnetization Enhancement (RIME) Contrast Agent for -Glucuronidase Activity Profiling. Inorg. Chem.51, 12426-12435.

14. Chen, P.-Y., K.-S. Huang, C.-C. Tsai, T.-P. Wang, and E.-C. Wang*. (2012) Pd(II)-Mediated Cyclization of o-Allylbenzaldehydes in Water: A Novel Synthesis of Isocoumarins. Org. Lett.14, 4930-4933.

15. Chen, P.-Y., H.-M. Chen, M. Y. Chiang, Y.-F. Wang, S-R. Li, T.-P. Wang, E.-C. Wang*. (2012) A novel and chemoselective synthesis of substituted 3,4-dihydroisoquinolin-1(2H)-ones from o-oxiranylmethylbenzonitrile intermediates and TBAB/NaCN. Tetrahedron68, 3030-3036.

16. Wang, T.-P.*, L.-L. Chang, S.-N. Chang, E.-C. Wang, L.-C. Hwang, Y.-H. Chen, and Y.-M. Wang (2012) Successful Preparation and Characterization of Biotechnological Grade Agarose from Indigenous Gelidium amansii of Taiwan. Process Biochem.47, 550–554.

17. Wang, T.-P.*, Y.-C. Su, Y. Chen, Y.-M. Liou, K.-L. Lin, E.-C. Wang, L.-C. Hwang, Y.-M. Wang, and Y.-H. Chen (2012) In vitro Selection and Characterization of a Novel Zn(II)-Dependent Phosphorothiolate Thiolesterase Ribozyme. Biochemistry51, 496-510.

18. Chen, B.-H.*, M.-H. Hung, J. Y.-F. Chen, H.-W. Chang, M.-L. Yu, L. Wan, F. J. Tsai, T.-P. Wang, T.-F. Fu, C.-C. Chiu (2012) Anti-allergic activity of grapeseed extract (GSE) on RBL-2H3 mast cells. Food Chem.132, 968-974.

19. Chen, P.-Y., T.-P. Wang, K.-S. Huang, C.-L. Kao, J.-C. Tsai, E.-C. Wang* (2011) tert-BuOK-mediated carbanioneyne intramolecular cyclization: synthesis of 2-substituted 3-benzylbenzofurans. Tetrahedron67, 9291-9297.

20. Chen, P.-Y., T.-P. Wang, M. Y. Chiang, K.-S. Huang, C.-C. Tzeng, Y.-L. Chen, E.-C. 18. Wang*. (2011) Environmentally benign syntheses of flavanones. Tetrahedron67, 4155-4160.

21. Chen, L.-Y., S.-R. Li, P.-Y. Chen, H.-C. Chang, T.-P. Wang, I.-L. Tsai, and E.-C. Wang*. (2010) 2,3-Dichloro-5,6-dicyanobenzoquinone (DDQ) mediated oxidation-dehydrogenation of 2-aroyl-3,4-dihydro-2H-benzopyrans : synthesis of 2-aroylbenzopyran-4-ones. ARKIVO, xi, 64-76.

22. Hwang, L.-C.*, Y.-C. Su, T.-P. Wang, L.-T. Liu, and G.-H. Lee (2010) Crystal structure of 3-amino-2-benzyl-6-bromo-1,2,4-triazin-5(2H)-one. X-ray Struct. Anal. Online, 26, 61-62.

23. Ko, C.-H.*, Z.-P. Lin, J. Tu, C.-H. Tsai, C.-C. Liu, H.-T. Chen, T.-P. Wang (2010) Xylanase production by Paenibacillus campinasensis BL11 and its pretreatment of hardwood kraft pulp bleaching. Int. Biodeterior. Biodegradation.64, 13–19.

24. Su, S.-H., W.-C. Tsai, C.-Y. Lin, W.-R. Lin, T.-C. Chen, P.-L. Lu, P.-M. Huang, J.-R. Tsai, Y.-L. Wang, M.-C. Feng, T.-P. Wang, Y.-H. Chen (2010) Clinical features and outcomes of spinal tuberculosis in southern Taiwan. J. Microbiol. Immunol. Infect., 43, 291–300.

25. Wang, T.-P.*, Y.-J. Chiou, Y. Chen, E.-C. Wang, L.-C. Hwang, B.-H. Chen, Y.-H. Chen, and C.-H. Ko (2010) Versatile phosphoramidation reactions for nucleic acid conjugations with peptides, proteins, chromophores, and biotin derivatives. Bioconjugate Chem. 21, 1642–1655.

26. Chen, L.-Y., S.-R. Li, P.-Y. Chen, I.-L. Tsai, C.-L. Hsu, H.-P. Lin, T.-P. Wang, E.-C. Wang* (2009) A novel carbanion-olefin intramolecular cyclization: synthesis of substituted 2-aroyl-3,4-dihydro-2H-benzopyrans from salicylaldehydes. Tetrahedron Lett., 50, 5748-5750.

 

 

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